Radiative-surface plasmon resonance for the detection of apolipoprotein E in medical diagnostics applications

• DOI: 10.1016/j.nano.2012.10.007
• Publication Date: 2012-11-21T20:15:36Z
• Authors: Beniamino Sciacca; Alexandre François; Manuela Klingler-...; Julie Brazzatti; Megan Penno; Peter Hoffmann; Tanya M. Monro
• Keywords: Biomolecule

Surface plasmon resonance (SPR)-based sensors enable the rapid, label-free and highly sensitive detection of a large range biomolecules. We have previously shown that, using silver-coated optical fibers with high surface roughness, re-scattering light from plasmons is possible, turning SPR into radiative process. The efficacy this platform has proven for biomolecules such as viruses, proteins enzymes. Here, we demonstrate that by bringing together novel emission-based fiber an improved functionalization process aimed at properly orienting antibodies, it possible to rapidly specifically detect regulation human apolipoprotein E (apoE), low-molecular-weight protein (~39 kDa) known be involved in cardiovascular diseases, Alzheimer's disease gastric cancer. results obtained clearly show new sensing potential serve tool point-of-decision medical diagnostics.In study, described. Properly antibodies on enables rapid specific (apoE).