Highly sensitive localized surface plasmon resonance immunosensor for label-free detection of HIV-1

• DOI: 10.1016/j.nano.2013.03.005
• Publication Date: 2013-03-16T08:39:33Z
• Authors: Jin-Ho Lee; Byung-Chan Kim; Byung-Keun Oh; Jeong-Woo Choi
• Keywords: Immunoassay; Optical Phenomena; Staining and Labeling; Virion; Reproducibility of Results; Enzyme-Linked Immunosorbent Assay; Genome, Viral; 02 engineering and technology; Surface Plasmon Resonance; 3. Good health; HEK293 Cells; Limit of Detection; HIV-1; Humans; Nanoparticles; RNA, Viral; Gold; 0210 nano-technology

A highly sensitive label-free immunosensor for the detection of HIV-1 is newly developed based on localized surface plasmon resonance (LSPR) method. Uniform nanopattern of circular Au-dots (10-20nm) was fabricated on indium tin oxide (ITO) coated glass substrate by simple electrochemical deposition method. The surface of Au nanopattern was modified with HIV-1 neutralizing gp120 monoclonal antibody fragments. The modified substrate was employed to measure various concentrations of HIV-1 particles quantitatively based on the shift of longitudinal wavelength in the UV-Vis spectrum which results from the changes of local refractive index induced by specific antigen-antibody recognition events. The detection limit of the HIV-1 particles was estimated to be 200fg/mL, which is 10 fold higher than that of previously reported virus detection method based on LSPR. Since fabricated LSPR immunosensor has high sensitivity and selectivity, it is a promising approach for biological/medical sample analysis and various kinds of virus detection.A localized surface plasmon resonance-based virus detection method is shown to have an order of magnitude improvement in detectable concentration of HIV-1 particles. Similar approaches may be used for screening other viral particles as well.