Nanostructured scaffolds based on bioresorbable polymers and graphene oxide induce the aligned migration and accelerate the neuronal differentiation of neural stem cells
0301 basic medicine
0303 health sciences
micro- and nanopatterning
Tissue Engineering
Tissue Scaffolds
Polymers
Nanofibers
biodegradable polymer
Cell Differentiation
migration
Nanostructures
cell differentiation
Mice
03 medical and health sciences
Neural Stem Cells
graphene oxide
Animals
Graphite
neural stem cells
DOI:
10.1016/j.nano.2020.102314
Publication Date:
2020-10-12T16:55:22Z
AUTHORS (10)
ABSTRACT
Within the field of neural tissue engineering, there is a huge need for the development of materials that promote the adhesion, aligned migration and differentiation of stem cells into neuronal and supportive glial cells. In this study, we have fabricated bioresorbable elastomeric scaffolds combining an ordered nanopatterned topography together with a surface functionalization with graphene oxide (GO) in mild conditions. These scaffolds allowed the attachment of murine neural stem cells (NSCs) without the need of any further coating of its surface with extracellular matrix adhesion proteins. The NSCs were able to give rise to both immature neurons and supporting glial cells over the nanostructured scaffolds in vitro, promoting their aligned migration in cell clusters following the nanostructured grooves. This system has the potential to reestablish spatially oriented neural precursor cell connectivity, constituting a promising tool for future cellular therapy including nerve tissue regeneration.
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CITATIONS (24)
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