Reduction in mitochondrial oxidative stress mediates hypoxia-induced resistance to cisplatin in human transitional cell carcinoma cells

• DOI: 10.1016/j.neo.2021.05.013
• Publication Date: 2021-06-13T21:30:18Z
• Authors: Myung-Chul Kim; Sung-Hyun Hwang; Yeseul Yang; Na-Yon Kim; Yongbaek Kim
• Keywords: Membrane Potential, Mitochondrial; 0301 basic medicine; Carcinoma, Transitional Cell; 0303 health sciences; mtDNA; Cell Survival; Neoplasms. Tumors. Oncology. Including cancer and carcinogens; Apoptosis; DNA Fragmentation; Models, Biological; Mitochondria; 3. Good health; Oxidative Stress; 03 medical and health sciences; Oxidative stress; Drug Resistance, Neoplasm; Drug resistance; Cell Line, Tumor; Humans; Tumor Hypoxia; Cisplatin; Hypoxia; Reactive Oxygen Species; RC254-282; Original Research

Tumor hypoxia is known to promote the acquisition of more aggressive phenotypes in human transitional cell carcinoma (TCC), including drug resistance. Accumulating evidence suggests that mitochondria play a central role in the chemoresistance of TCC. However, the role of mitochondria in the hypoxia-induced drug resistance in TCC remains elusive. The present study investigated the function of mitochondria in the drug resistance using a TCC cell line under hypoxic conditions. In vitro hypoxia (0.1% O2, 48 h) was achieved by incubating TCC cells in air chamber. Mitochondrial events involving hypoxia-induced drug resistance were assessed. Hypoxia significantly reduced the cisplatin-induced apoptosis of TCC cells. Additionally, hypoxia substantially decreased the level of mitochondrial reactive oxygen species (ROS) generated by cisplatin treatment. Analogously, elimination of mitochondrial ROS significantly rescued cells from cisplatin-induced apoptosis. Hypoxia enhanced mitochondrial hyperpolarization, which was not related to ATP production or the reversal of ATP synthase activity. The mitochondrial DNA (mtDNA) amplification efficiency data illustrated that hypoxia significantly prevented oxidative damage to the mitogenome. Moreover, transmission electron microscopy revealed that cisplatin-induced disruption of the mitochondrial ultrastructure was abated under hypoxic conditions. Notably, depletion of mtDNA by ethidium bromide abrogated hypoxia-induced resistance to cisplatin. Taken together, the present study demonstrated that TCC cells exposed to hypoxic conditions rendered mitochondria less sensitive to oxidative stress induced by cisplatin treatment, leading to enhanced drug resistance.