The breast tissue microbiome, stroma, immune cells and breast cancer

• DOI: 10.1016/j.neo.2022.100786
• Publication Date: 2022-03-30T21:34:21Z
• Authors: Tina J Hieken; Jun Chen; Beiyun Chen; Stephen Johnson; Tanya L Hoskin; Amy C Degnim; Marina R Walther-...; Nicholas Chia
• Keywords: UniFrac

Stromal and immune cell composition alterations in benign breast tissue associate with future cancer risk. Pilot data suggest the innate microbiome of normal differs between women without cancer. Microbiome might explain microenvironment variations associated disease status.Prospectively-collected sterile tissues from (n=16) or malignant (n=17) underwent 16SrRNA sequencing Illumina MiSeq Hybrid-denovo pipeline processing. Breast was scored for fibrosis fat percentages infiltrates (lobulitis) classified as absent/mild/moderate/severe. Alpha beta diversity were calculated on rarefied OTU associations analyzed multiple linear regression PERMANOVA.Breast stromal fat% lower fibrosis% higher versus (median 30% 60%, p=0.01, 70% 30%, p=0.002, respectively). The varied composition. (Chao1) correlated (r=0.38, p=0.02) (r=-0.32, p=0.05) different microbial populations indicated by metrics (weighted UniFrac, p=0.08, fat%, p=0.07, fibrosis%). Permutation testing FDR control revealed taxa differences Firmicutes, Bacilli, Bacillales, Staphylococcaceae genus Staphylococcus, Spirochaetes, Spirochaetales, Proteobacteria RF32, Sphingomonadales, Staphylococcaceae, genera Clostridium, Actinobacteria Adlercreutzia. Moderate/severe lobulitis more common (73%) than (13%), p=0.003, but no significant seen.These a link its microbiome, further supporting connection