Treatment of hypoxic–ischemic encephalopathy in mouse by transplantation of embryonic stem cell-derived cells

Brain Infarction Cerebral Cortex Neurons 0301 basic medicine Memory Disorders 0303 health sciences Graft Survival Cell Differentiation Nerve Tissue Proteins Hippocampus Cell Line Mice, Inbred C57BL Nestin Mice 03 medical and health sciences Animals, Newborn Intermediate Filament Proteins Hypoxia-Ischemia, Brain Nerve Degeneration Animals Microtubule-Associated Proteins Biomarkers Embryonic Stem Cells
DOI: 10.1016/j.neuint.2007.04.012 Publication Date: 2007-04-30T07:13:15Z
ABSTRACT
A 7-day-old hypoxic-ischemic encephalopathy (HIE) mouse model was used to study the effect of transplantation of embryonic stem (ES) cell-derived cells on the HIE. After the inducement in vitro, the ES cell-derived cells expressed Nestin and MAP-2, rather than GFAP mRNA. After transplantation, ES cell-derived cells can survive, migrate into the injury site, and specifically differentiate into neurons, showing improvement of the learning ability and memory of the HIE mouse at 8 months post-transplantation. The non-grafted HIE mouse brain showed typical pathological changes in the hippocampus and cerebral cortex, where the number of neurons was reduced, while in the cell graft group, number of the neurons increased in the same regions. Although further study is necessary to elucidate the precise mechanisms responsible for this functional recovery, we believe that ES cells have advantages for use as a donor source in HIE.
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