The Oriented Emergence of Axons from Retinal Ganglion Cells Is Directed by Laminin Contact In Vivo

• DOI: 10.1016/j.neuron.2011.03.013
• Publication Date: 2011-04-30T18:26:17Z
• Authors: Owen Randlett; Lucia Poggi; Flavio R. Zolessi; William A. Harris
• Keywords: Growth cone

How the site of axon emergence is specified during neural development not understood. Previous studies disagree on relative importance intrinsic and extrinsic mechanisms. The axons retinal ganglion cells (RGCs) emerge basally in vivo, yet because RGCs develop from polarized neuroepithelial within a environment, disentangling influences challenge. We use time-lapse imaging to demonstrate that Laminin acting directly necessary sufficient orient vivo. contact with basal processes newborn prevents entering stochastic Stage 2 phase, directs rapid accumulation early axonal marker Kif5c560-YFP, leads formation growth cones. These results suggest contact-mediated cues may be critical for account differences cellular behavior observed vitro vivo.Video AbstracteyJraWQiOiI4ZjUxYWNhY2IzYjhiNjNlNzFlYmIzYWFmYTU5NmZmYyIsImFsZyI6IlJTMjU2In0.eyJzdWIiOiI3MWNjY2JhZTFjMmMxZWIyZTU1NDlmNmY4MzU5MjJjYyIsImtpZCI6IjhmNTFhY2FjYjNiOGI2M2U3MWViYjNhYWZhNTk2ZmZjIiwiZXhwIjoxNjcyMzUyMjgwfQ.soGq8o9QwHunWKPokVBaGbO4aiL2IdH8KCPedCELesfuUo2DWXLEkTdwJ5LCOaUJtsyEVEtMZ14kz_L3vot_1TVggok4Q8AiTG8VhsaHF-4ZAvmpdG0eTNF77PZiVBTRq1hcgtmtg8JMbIvuTtPopBtfqNnbAsmy4OwM6jvhiqbWRCxtMcWbvzjMLlXNKhfBfLm68NFUyfDwHgyOitBf6iqL63kAiw6Pw83Ag4zp12r725lggIhQ6_AtUGT8E1-fyYzI1dKUH-URQQCCiUhu68JXcSWsa0NlTxxbn29NwBg8nYNHDMDJYhyEmwyxTiC1xkNz1QJnLC51VkVYJEUahw(mp4, (15.99 MB) Download video