Amplification of Drosophila Olfactory Responses by a DEG/ENaC Channel

• DOI: 10.1016/j.neuron.2019.08.041
• Publication Date: 2019-10-16T14:27:31Z
• Authors: Renny Ng; Secilia S. Salem; Shiuan-Tze Wu; Meilin Wu; Hui-Hao Lin; Andrew K. Shepherd; William J. Joiner; Jing W. Wang; Chih-Ying Su
• Keywords: Male; 1.1 Normal biological development and functioning; Sexual Behavior; Olfactory Receptor Neurons; Sodium Channels; Or47b; DEG/ENaC; Sexual Behavior, Animal; Underpinning research; Psychology; Animals; Drosophila Proteins; PPK25; Neurology & Neurosurgery; Animal; juvenile hormone; Neurosciences; Courtship; courtship behavior; olfactoy receptor neurons; Smell; Drosophila melanogaster; Ir84a; ionotropic signaling; Cognitive Sciences; insect olfactory amplification

Insect olfactory receptors operate as ligand-gated ion channels that directly transduce odor stimuli into electrical signals. However, in the absence of any known intermediate transduction steps, it remains unclear whether and how these ionotropic inputs are amplified in olfactory receptor neurons (ORNs). Here, we find that amplification occurs in the Drosophila courtship-promoting ORNs through Pickpocket 25 (PPK25), a member of the degenerin/epithelial sodium channel family (DEG/ENaC). Pharmacological and genetic manipulations indicate that, in Or47b and Ir84a ORNs, PPK25 mediates Ca2+-dependent signal amplification via an intracellular calmodulin-binding motif. Additionally, hormonal signaling upregulates PPK25 expression to determine the degree of amplification, with striking effects on male courtship. Together, these findings advance our understanding of sensory neurobiology by identifying an amplification mechanism compatible with ionotropic signaling. Moreover, this study offers new insights into DEG/ENaC activation by highlighting a novel means of regulation that is likely conserved across species.