Transient and Persistent Representations of Odor Value in Prefrontal Cortex

• DOI: 10.1016/j.neuron.2020.07.033
• Publication Date: 2020-08-21T14:32:57Z
• Authors: Peter Y. Wang; Cristian Boboila; Matthew Chin; Alexandra Higashi...; Philip Shamash; Zheng Wu; Nicole P. Stein; L.F. Abbott; Richard Axel
• Keywords: Neurons; 0301 basic medicine; Appetitive Behavior; Intravital Microscopy; Conditioning, Classical; Association Learning; Prefrontal Cortex; Piriform Cortex; Olfactory Pathways; Optogenetics; Mice; 03 medical and health sciences; Microscopy, Fluorescence, Multiphoton; Odorants; Animals

The representation of odor in olfactory cortex (piriform) is distributive and unstructured and can only be afforded behavioral significance upon learning. We performed 2-photon imaging to examine the representation of odors in piriform and in two downstream areas, the orbitofrontal cortex (OFC) and the medial prefrontal cortex (mPFC), as mice learned olfactory associations. In piriform, we observed that odor responses were largely unchanged during learning. In OFC, 30% of the neurons acquired robust responses to conditioned stimuli (CS+) after learning, and these responses were gated by internal state and task context. Moreover, direct projections from piriform to OFC can be entrained to elicit learned olfactory behavior. CS+ responses in OFC diminished with continued training, whereas persistent representations of both CS+ and CS- odors emerged in mPFC. Optogenetic silencing indicates that these two brain structures function sequentially to consolidate the learning of appetitive associations.