Somatostatin receptor-mediated suppression of gabaergic synaptic transmission in cultured rat retinal amacrine cells

• DOI: 10.1016/j.neuroscience.2014.05.013
• Publication Date: 2014-05-16T00:01:18Z
• Authors: W. Chen; J.-B. Ke; H.-J. Wu; Y. Miao; F. Li; X.-L. Yang; Z. Wang
• Keywords: 0301 basic medicine; Patch-Clamp Techniques; Presynaptic Terminals; Cyclic AMP-Dependent Protein Kinases; Synaptic Transmission; 3. Good health; Rats, Sprague-Dawley; 03 medical and health sciences; Amacrine Cells; Inhibitory Postsynaptic Potentials; Cyclic AMP; Animals; Inositol 1,4,5-Trisphosphate Receptors; Calcium Channels; Receptors, Somatostatin; Somatostatin; Cells, Cultured; Protein Kinase C; gamma-Aminobutyric Acid; Adenylyl Cyclases; Signal Transduction

Somatostatin (SRIF) modulates neurotransmitter release by activating the specific receptors (sst1-sst5). Our previous study showed that sst5 receptors are expressed in rat retinal GABAergic amacrine cells. Here, we investigated modulation of GABA release by SRIF in cultured amacrine cells, using patch-clamp techniques. The frequency of spontaneous inhibitory postsynaptic currents (sIPSCs) in the amacrine cells was significantly reduced by SRIF, which was partially reversed by BIM 23056, an sst5 receptor antagonist, and was further rescued by addition of CYN-154806, an sst2 receptor antagonist. Both nimodipine, an L-type Ca2+ channel blocker, and ω-conotoxin GVIA, an N-type Ca2+ channel blocker, suppressed the sIPSC frequency, and in the presence of nimodipine and ω-conotoxin GVIA, SRIF failed to further suppress the sIPSC frequency. Extracellular application of forskolin, an activator of adenylate cyclase, increased the sIPSC frequency, while the membrane permeable protein kinase A (PKA) inhibitor Rp-cAMP reduced it, and in the presence of Rp-cAMP, SRIF did not change sIPSCs. However, SRIF persisted to suppress the sIPSCs in the presence of KT5823, a protein kinase G (PKG) inhibitor. Moreover, pre-incubation with Bis IV, a protein kinase C (PKC) inhibitor, or pre-application of xestospongin C, an inositol 1,4,5-trisphosphate receptor (IP3R) inhibitor, SRIF still suppressed the sIPSC frequency. All these results suggest that SRIF suppresses GABA release from the amacrine cells by inhibiting presynaptic Ca2+ channels, in part through activating sst5/sst2 receptors, a process that is mediated by the intracellular cAMP-PKA signaling pathway.