Transplantation of Human Umbilical Cord Blood Mononuclear Cells Attenuated Ischemic Injury in MCAO Rats via Inhibition of NF-κB and NLRP3 Inflammasome

• DOI: 10.1016/j.neuroscience.2017.11.027
• Publication Date: 2017-11-24T13:31:30Z
• Authors: Lu Liu; Juan Cen; Yong Man; Jianbin Li; Dahuan Zhang; Fei Wang; Jinhua Li; Ji Ma; Xiaoming Wang; Biansheng Ji
• Keywords: Adult; Male; 0303 health sciences; Inflammasomes; Caspase 1; Interleukin-1beta; NF-kappa B; Brain; Apoptosis; Infarction, Middle Cerebral Artery; Rats, Sprague-Dawley; Disease Models, Animal; Random Allocation; Young Adult; 03 medical and health sciences; NLR Family, Pyrin Domain-Containing 3 Protein; Leukocytes, Mononuclear; Animals; Humans; Cord Blood Stem Cell Transplantation

Accumulated evidence displayed that transplantation of stem cells may be a promising approach for the treatment of neurological disorders. However, the underlying mechanisms remain to be well elucidated. Moreover, some investigators cannot reproduce similar results as the previous. The present results showed that transplantation of fresh human umbilical cord blood mononuclear cells (cbMNCs) attenuated ischemic damage in middle cerebral artery occlusion (MCAO) rats, accompanied with improvement of neurologic deficits, learning and memory function. The increase in neovascularization and related molecules such as vascular endothelial growth factor (VEGF), Angiopoietin-1 (Ang-1) and endothelium-specific receptor tyrosine kinase 2 (Tie-2) in the injured brain was observed in cbMNCs-treated rats. Moreover, nuclear factor-κB (NF-κB) activation and nucleotide binding and oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome were also inhibited by the cells graft, resulting in reduction in cleaved caspase-1 and mature interleukin-1β (IL-1β) content. These results suggested that the protective actions of the cells on the cerebral ischemia may be related to inhibition of NF-κB pathway and NLRP3 inflammasome.