Minocycline Preserves the Integrity and Permeability of BBB by Altering the Activity of DKK1–Wnt Signaling in ICH Model

• DOI: 10.1016/j.neuroscience.2019.06.038
• Publication Date: 2019-07-22T19:34:42Z
• Authors: Guoqing Wang; Zhihua Li; Shujian Li; Junling Ren; Vigneyshwar Suresh; Dingkang Xu; Weidong Zang; Xianzhi Liu; Wei Li; Huizhi Wang; Fuyou Guo
• Keywords: Minocycline; Evans Blue; Occludin; Extravasation

Disruption of the blood-brain barrier (BBB) and subsequent neurological deficits are most severe consequence intracerebral hemorrhage (ICH). Minocycline has been wildly used clinically as a protective agent in clinical practice. However, underlying mechanisms by which minocycline functions remain unclear. Therefore, we assessed influence on BBB structure, function, inflammatory responses collagenase-induced ICH model, elucidated molecular well. Following single injection collagenase VII-S into basal ganglia, integrity was Evans blue extravasation while function using an established neurologic scoring system. treatment significantly alleviated severity disruption, brain edema, model. Moreover, decreased production mediators including TNF, IL-6, MMP-9, microglia. DKK1 expression but increased Wnt1, β-catenin Occludin, phenomenon mimicked silencing. These data suggest that improves consequences preserving attenuating DKK1-related manner involves enhancement Wnt1-β-catenin activity.