Testis electroporation coupled with autophagy inhibitor to treat non-obstructive azoospermia

• DOI: 10.1016/j.omtn.2022.10.022
• Publication Date: 2022-11-10T01:11:23Z
• Authors: Liying Wang; Chao Liu; Huafang Wei; Yingchun Ouyang; Mingzhe Dong; Ruidan Zhang; Lina Wang; Yinghong Chen; Yanjie Ma; Mengmeng Guo; Yang Yu; Qing-Yuan Sun; Wei Li
• Keywords: electroporation; 0301 basic medicine; autophagy; 03 medical and health sciences; MT: Delivery Strategies; 3-methyladenine; non-obstructive azoospermia; Original Article; Therapeutics. Pharmacology; RM1-950; male infertility; 3. Good health

Infertility affects 10%-20% of the population in most countries, with approximately half those cases resulting from male infertility. Although millions infertile men are able to have children assistance reproductive technology, individuals non-obstructive azoospermia (NOA) syndrome unable do so because they lack functional sperm. Therefore, some other strategies for NOA still urgently needed. Our current study uses an NOA-like mouse model optimize microinjection and a subsequent electroporation method test potential treatment strategies. We showed that spermatogenetic process could be partially rescued young Stra8-Rnf20 -/- mice Rnf20 plasmids into testes. All meiotic prophase I stages identified, programmed DNA double-strand break repair factors successfully recruited spermatocytes after electroporation. Moreover, by including autophagy inhibitor treatment, significantly improved rescue efficiency adult mice. Most importantly, infertility caused depletions overcome coupled intracytoplasmic sperm injection. studies establish relative safe efficient testis system provide promising therapeutic strategy patients NOA.