Development of new non-viral systems for genetic modification of senescent cells

• DOI: 10.1016/j.omtn.2023.03.010
• Publication Date: 2023-03-21T08:21:41Z
• Authors: Junquera López-Se...; Diego Miranda-Bal...; Alba Iglesias-Fente; Marta Sacristán-S...; Natalia Carballo-...; María C. Arufe; Ana Rey-Rico; Juan Fafián-Labora
• Keywords: Lipofectamine; Niosome; Senescence

Senescence is a process characterized by prolonged irreversible cell-cycle arrest. The accumulation of senescent cells in tissues related to aging and the development age-related diseases. Recently, gene therapy has emerged as powerful tool for treating age-associated diseases transference specific genes into target cell population. However, high sensitivity significantly precludes their genetic modification via classical viral non-viral systems. Niosomes are self-assembled nanocarriers that exhibit important advantages due elevated cytocompatibility, versatility, cost-efficiency, arising new alternative cells. In this work, we explore first time use niosomes umbilical cord-derived mesenchymal stem We report niosome composition greatly affected transfection efficiency; those formulations prepared medium with sucrose containing cholesterol helper lipid being most suitable transfect Moreover, resulting exhibited superior efficiency markedly less cytotoxicity than commercial reagent Lipofectamine. These findings highlight potentiality effective vectors cells, providing tools prevention and/or treatment