A chlorin e6 derivative-mediated photodynamic therapy inhibits cutaneous squamous cell carcinoma cell proliferation via Akt/mTOR signaling pathway

• DOI: 10.1016/j.pdpdt.2023.103332
• Publication Date: 2023-02-14T07:46:50Z
• Authors: Hui Tao; Haiyan Zhang; Detian Xu; Guorong Yan; Yuhao Wu; Guolong Zhang; Qingyu Zeng; Xiuli Wang
• Keywords: 0301 basic medicine; Photosensitizing Agents; Skin Neoplasms; Porphyrins; TOR Serine-Threonine Kinases; 3. Good health; 03 medical and health sciences; Photochemotherapy; Cell Line, Tumor; Carcinoma, Squamous Cell; Animals; Proto-Oncogene Proteins c-akt; Signal Transduction; Cell Proliferation

Although most cutaneous squamous cell carcinoma (cSCC) cases are generally nonlethal and manageable with surgical excision, there ares till significant hazards for patients who are ineligible for surgical resection. We sought to find a suitable and effective treatment for cSCC.We modified chlorin e6 by adding a hydrogen chain with a six-carbon ring to the benzene ring and named this new photosensitizer as STBF. We first investigated the fluorescence characteristics, cellular uptake of STBF and subcellular localization. Next, cell viability was detected by CCK-8 assay and the TUNEL staining was performed. Akt/mTOR-related proteins were examined by western blot.STBF-photodynamic therapy (PDT) inhibits cSCC cells viability in a light dose dependent manner. The antitumor mechanism of STBF-PDT might be due to the suppression of the Akt/mTOR signaling pathway. Further animal investigation determined that STBF-PDT led to a marked reduction in tumor growth.Our results suggest that STBF-PDT exerts significant therapeutic effects in cSCC. Thus, STBF-PDT is expected to be a promising method for the treatment of cSCC and the photosensitizer STBF may be destined for a wider range of applications in photodynamic therapy.