Characterization of contryphans from Conus loroisii and Conus amadis that target calcium channels

• DOI: 10.1016/j.peptides.2006.07.009
• Publication Date: 2006-09-13T11:16:35Z
• Authors: V. Sabareesh; K. Hanumae Gowd; P. Ramasamy; S. Sudarslal; K.S. Krishnan; S.K. Sikdar; P. Balaram
• Keywords: Neurons; 0301 basic medicine; 0303 health sciences; Patch-Clamp Techniques; Molecular Sequence Data; Conus Snail; Molecular Biophysics Unit; Peptides, Cyclic; Rats; 03 medical and health sciences; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Animals; Amino Acid Sequence; Calcium Channels; Rats, Wistar; Conotoxins; Peptides; Cells, Cultured

Distinctly different effects of two closely related contryphans have been demonstrated on voltage-activated Ca(2+) channels. The peptides Lo959 and Am975 were isolated from Conus loroisii, a vermivorous marine snail and Conus amadis, a molluscivore, respectively. The sequences of Lo959 and Am975 were deduced by mass spectrometric sequencing (MALDI-MS/MS) and confirmed by chemical synthesis. The sequences of Lo959, GCP(D)WDPWC-NH(2) and Am975, GCO(D)WDPWC-NH(2) (O: 4-trans-hydroxyproline: Hyp), differ only at residue 3; Pro in Lo959, Hyp in Am975, which is identical to contryphan-P, previously isolated from Conus purpurascens, a piscivore; while Lo959 is a novel peptide. Both Lo959 and Am975 undergo slow conformational interconversion under reverse-phase chromatographic conditions, a characteristic feature of all contryphans reported thus far. Electrophysiological studies performed using dorsal root ganglion neurons reveal that both peptides target high voltage-activated Ca(2+) channels. While Lo959 increases the Ca(2+) current, Am975 causes inhibition. The results establish that subtle sequence effects, which accompany post-translational modifications in Conus peptides, can have dramatic effects on target ion channels.