Baicalin restore intestinal damage after early-life antibiotic therapy: the role of the MAPK signaling pathway

• DOI: 10.1016/j.phrs.2024.107194
• Publication Date: 2024-04-23T15:29:48Z
• Authors: Shunfen Zhang; Shanlong Tang; Zhengqun Liu; Huiyuan Lv; Xueying Cai; Ruqing Zhong; Liang Chen; Hongfu Zhang
• Keywords: Baicalin; Lincomycin; Dysbiosis

Antibiotic related intestinal injury in early life affects subsequent health and susceptibility. Here, we employed weaned piglets as a model to investigate the protective effects of baicalin against early-life antibiotic exposure-induced microbial dysbiosis. Piglets exposed lincomycin showed marked reduction body weight (p < 0.05) deterioration jejunum morphology, alongside an increase antibiotic-resistant bacteria such Staphylococcus, Dolosicoccus, Escherichia-Shigella, Raoultella. In contrast, treatment resulted weights, profiles that closely resembled those control group > 0.05), with significant norank_f_Muribaculaceae Prevotellaceae_NK3B31_group colonization compared 0.05). Further analysis through fecal transplantation into mice revealed exposure led alterations morphology composition, notably increasing harmful microbes decreasing beneficial ones norank_Muribaculaceae Akkermansia This shift was associated metabolites disruption calcium signaling pathway gene expression. Conversely, supplementation not only counteracted these but also enhanced regulated genes within MAPK (MAP3K11, MAP4K2, MAPK7, MAPK13) channel proteins (ORA13, CACNA1S, CACNA1F CACNG8), suggesting mechanism which mitigates antibiotic-induced disturbances. These findings highlight baicalin's potential plant extract-based intervention for preventing antibiotic-related offer new targets therapeutic strategies.