Acute and subacute toxicity study of ethanolic extract of Calotropis procera (Aiton) Dryand flower in Swiss albino mice

• DOI: 10.1016/j.phyplu.2022.100224
• Publication Date: 2022-01-17T07:00:04Z
• Authors: Ashutosh Kumar; Brijesh Kumar; Rajesh Kumar; Ajay Kumar; Manish Singh; Vinod Tiwari; Anshuman Trigunayat; Paramita Paul; Pratistha Singh
• Keywords: Calotropis procera; Procera

Calotropis procera is a large shrub which consists many medicinal properties, used in treatment of snake bite, sinus fistula, rheumatism, mumps, burn injuries, inflammation and jaundice traditionally. All the parts were utilized diseases out leaves roots investigated for its toxicity profile that showed dose dependent toxicity. Toxicity flowers was not previous studies. The aim this study to explore acute subacute ethanolic extract safe use traditional medicine. In toxicity, total 20 female mice (Swiss albino), weighing between 23 32 g randomly divided into four experimental groups: control, 300, 1000, 2000 mg/kg groups with 5 each, each received single at or mg/kg, respectively. Animals monitored 14 days. study, 40 (23–32 g) 4 groups, containing males females. Group 1 (control group) vehicle 2, 3, doses 300 mg/Kg, 1000 mg/Kg b.w., respectively, 28 consecutive conducted compliance OECD guidelines 407 423. Acute no mortality mg/Kg. statistical analysis hematological biochemical parameters significant differences compared control group except marked increase segmented neutrophils. Histopathological studies revealed structural among treated comparison group. It concluded oral administration flower, administered acutely, did cause any notable changes Therefore, approximate lethal (ALD) higher than 2,000 mg/kg. 28-day model, mortality, treatment-related observed body weight, organ blood analysis, histopathologic examinations These findings indicate no-observed-adverse-effect-level (NOAEL) flower greater mg/kg/day.