Isolation and bioactivity of diterpenoids from the roots of Salvia grandifolia

• DOI: 10.1016/j.phytochem.2015.03.014
• Publication Date: 2015-04-25T15:36:17Z
• Authors: Jie Kang; Li Li; Dingding Wang; Hongqing Wang; Chao Liu; Baoming Li; Yu Yan; Lianhua Fang; Guanhua Du; Ruoyun Chen
• Keywords: Molecular Structure; Vasodilator Agents; Molecular Conformation; Crystallography, X-Ray; Antineoplastic Agents, Phytogenic; Plant Roots; 01 natural sciences; Rats; 0104 chemical sciences; Abietanes; Animals; Salvia; Diterpenes; Nuclear Magnetic Resonance, Biomolecular; Drugs, Chinese Herbal

A phytochemical investigation of root extracts of Salvia grandifolia led to isolation of six previously unreported diterpenoids, grandifolias A-F, along with eight known compounds. The structures of grandifolias A-F were primarily established by extensive 1D and 2D NMR spectroscopic analyses, as well as HRESIMS data. Their absolute configurations were assigned by their calculated and experimental electronic circular dichroism spectra or by X-ray diffraction analysis. All of the diterpenoids were evaluated for their vasorelaxant effects. Grandifolia B and isograndifoliol both exhibited dose-dependent vasorelaxant effects on rat aortic rings, preconstricted by KCl or norepinephrine, with EC50 values of 36.36-74.51μg/mL.