Cytotoxic and anti-angiogenic effects of lanostane triterpenoids from Ganoderma lucidum

• DOI: 10.1016/j.phytol.2015.02.012
• Publication Date: 2015-02-27T22:51:42Z
• Authors: Van Thu Nguyen; Nguyen The Tung; To Dao Cuong; Tran Manh Hung; Jeong Ah Kim; Mi Hee Woo; Jae Sue Choi; Jeong-Hyung Lee; Byung Sun Min
• Keywords: Lanostane; Polyporaceae; Terpene

Abstract Two new lanostane triterpenes, 3α,12β,15α-triacetoxy-5α-lanosta-7,9(11),24-trien-26-oic acid (1) and 5α-lanosta-8,24-diene-26,27-dihydroxy-3,7-dione (2), together with sixteen known compounds (3–18) were isolated from the fruiting bodies of the Vietnamese mushroom Ganoderma lucidum. Their chemical structures were determined by extensive spectroscopic (IR, HR-EI-MS, 1D and 2D NMR) analyses. Potential cytotoxic activities of these compounds were evaluated against human non-small cell lung adenocarcinoma (A549), breast adenocarcinoma (MCF-7), and prostatic small cell carcinoma (PC-3). Among the compounds, 3α,12β,15α-triacetoxy-5α-lanosta-7,9(11),24-trien-26-oic acid (1) showed significant cytotoxic activity against PC-3 cells with an IC50 of 11.5 μM. In studies of anti-angiogenesis activity, ganoderic acid F (17) was found to have the most potent inhibitory effect on the formation of capillary-like structures of human umbilical vein endothelial cells.