PCR Product Detector with LED-Photodiode Fluorescence Sensing in a Nanoliter Flow-Cell for the High-Throughput Detection of Double-Stranded DNA

• DOI: 10.1016/j.proeng.2011.12.230
• Publication Date: 2012-01-09T20:06:53Z
• Authors: Johannes R. Peham; Michael J. Vellekoop; Christa Nöhammer; Herbert Wiesinger...
• Keywords: Photodiode

We present an endpoint PCR product sensing system for online detection of double-stranded DNA (dsDNA) down to 1 ng/μl in a flow-through design with throughput up 144 samples per hour. The device is assembled from off- the-shelf microfluidic and optical components chamber volume 520 nl without any lenses. Fluorescence realized LED-Photodiode combination, which makes this practicable every lab. direct combination continuous-flow realises integrated diagnostic pathogen detection.