Broiler chickens with 1950s genetics display a stable immune profile as measured by Kinome, mRNA expression, and metabolism when stimulated early in life with CpG

• DOI: 10.1016/j.psj.2022.101775
• Publication Date: 2022-02-11T06:56:43Z
• Authors: Bridget A. Aylward; Casey N. Johnson; Famatta Perry; Rose Whelan; Chi Zhang; Ryan J. Arsenault
• Keywords: Kinome; TLR9

Significant changes in growth potential and feed conversion have been bred into the modern broiler chicken for well over 60 yr. These metabolic had significant effects on immune performance as well. To better understand these genetic differences immunometabolism we studied response of Athens Canadian Random Bred (ACRB) heritage strain. We injected newly hatched ACRB chicks intraabdominally with CpG oligonucleotide, an immunostimulatory synthetic oligonucleotide. conducted species-specific kinome array analysis gene expression jejunum cecal tonsil tissue. also performed blood cells. In birds, there is initial inflammatory to injection at d 3 post-hatch activation PI3K-Akt, JAK-STAT, NF-κB signaling, IL-1β IL-6 mRNA expression. By 15 this changed deactivation downregulation responses but not broilers. Metabolic showed increase glycolysis peripheral mononuclear cells from birds given CpG, no difference ACRB. results show that may a less more stable profile stimulation than broilers, possibly resulting disease resistant phenotype overall.