In ovo probiotic supplementation promotes muscle growth and development in broiler embryos

• DOI: 10.1016/j.psj.2023.102744
• Publication Date: 2023-05-03T05:52:52Z
• Authors: Muhammed Shafeekh...; Maya Schlesinger; Yuying Ren; Mairui Gao; Amanda Liefeld; Sarah Reed; Mary Anne Amalara...
• Keywords: In ovo

In chickens, muscle development during embryonic growth is predominantly by myofiber hyperplasia. Following hatch, primarily occurs via hypertrophy of the existing myofibers. Since number set at production more fibers would provide a greater hatch and potential for posthatch hypertrophy. Therefore, to improve performance in broilers, this study investigated effect ovo spray application probiotics on overall morphometry broiler embryos. For study, fertile Ross 308 eggs were sprayed with different probiotics; Lactobacillus paracasei DUP 13076 (LP) L. rhamnosus NRRL B 442 (LR) prior incubation. The embryos sacrificed d 7, 10, 14, 18 embryo pectoralis major (PMM) sampling. Muscle sections stained imaged quantify fiber density (MFD), cross-sectional area (CSA), nuclei density. Additionally, gene expression assays performed elucidate myogenic genes. probiotic supplementation was found significantly weight, breast leg weight (P < 0.05). Further, histological analysis PMM revealed significant increase MFD probiotic-treated when compared control 18-day-old embryos, myofibers treatment group had smaller CSA (LP: 95.27 ± 3.28 μm2, LR: 178.84 15.1 μm2) (211.41 15.67 μm2). This decrease be associated concomitant (fibers/mm2) LP (13,647 482.15) LR (13,957 463.13) (7,680 406.78). myofibrillar hyperplasia groups upregulation key genes regulating including MYF5, MYOD, MYOG, IGF-1. summary, promoted broilers.