Simultaneous and rapid detection of avian respiratory diseases of small poultry using multiplex reverse transcription-Polymerase Chain Reaction assay

• DOI: 10.1016/j.psj.2023.102852
• Publication Date: 2023-06-08T02:38:09Z
• Authors: Tohid Piri-Gharaghie; Ghazal Ghajari; Naz Tavakoli Lahi...; Renzon Daniel Cos...; Fahdil Hussam; Roxana Yolanda Ca...; Mona Aghassizadeh...
• Keywords: Multiplex; Newcastle Disease; Avian infectious bronchitis; Infectious bursal disease

Major viral infections, such as Newcastle disease virus, infectious bronchitis avian influenza and bursal inflict significant injury to small poultry tremendous economic damage the sector. This research aims develop a multiplex reverse transcriptase polymerase chain reaction (m-RT-PCR) approach simultaneously determine these important pathogens. The conserved segment of various genetic sequences was used design synthesize specific primers. Moreover, positive controls, recombinant vectors were synthesized in this investigation. d-optimal improve PCR conditions Positive controls clinical samples assess m-PCR assay's specificity, sensitivity, repeatability, reproducibility. According sensitivity test findings, technique could generate 8 target genes from genomes using 1 × 102. In addition, pathogens detected infected samples. findings also suggest that live animal oral swabs not significantly different tissue sampling dead (P < 0.05), kit had high for analyzing both types suggested may detect evaluate infection birds with excellent throughput.