High rate of multidrug resistance and integrons in Escherichia coli isolates from diseased ducks in select regions of China

• DOI: 10.1016/j.psj.2023.102956
• Publication Date: 2023-07-25T08:17:28Z
• Authors: Shaqiu Zhang; Yanxi Shu; Yuwei Wang; Zhijun Zhong; Mingshu Wang; Renyong Jia; Shun Chen; Mafeng Liu; Dekang Zhu; Xinxin Zhao; Ying Wu; Qiao Yang; Juan Huang; Xumin Ou; Sai Mao; Qun Gao; Di Sun; Bin Tian; Anchun Cheng
• Keywords: Sulfamethoxazole; Resistome

With the increasing number of ducks being raised and consumed, it is crucial to monitor presence multidrug resistant (MDR) bacteria in duck farming. Waterfowl, such as ducks, can contribute rapid dissemination antibiotic resistance genes (ARGs). The objective this study was investigate antimicrobial (AMR), ARGs, mobile genetic elements (MGEs), IS26, tbrC, ISEcp1 Escherichia coli(E. coli) isolated from intestinal contents diseased between 2021 2022 Sichuan, Chongqing Anhui, China. AMR phenotypes 201 E. coli strains were determined using minimum inhibitory concentrations (MICs) method. Subsequently, polymerase chain reaction sequencing techniques employed screen for integron-integrase (intI1, intI2, intI3 genes), gene cassettes (GCs), MGEs, ARGs. results demonstrated that 96.5% isolates at least 1 antibiotic, with 88.1% displaying MDR phenotype. highest phenotype observed trimethoprim-sulfamethoxazole (88.1%). Furthermore, class 2 integrons detected 68.2% 3.0% all isolates, respectively, whereas no 3 found. Ten types GCs identified variable regions integrons. Moreover, 10 MGEs 46 combinations, IS26 exhibiting detection rate (89.6%). Among 22 tetA (77.1%) most frequently detected. In conjugational transfer experiment, transconjugants found carry specific ARGs their MIC values significantly higher than those recipient J53, indicating status bacteria. This emphasizes necessity monitoring farms. It provides valuable insights into complex formation mechanisms may aid preventing controlling spread waterfowl breeding farm.