Modified rougan decoction attenuates hepatocyte apoptosis through ameliorating mitochondrial dysfunction by upregulated SIRT1/PGC-1α signaling pathway

• DOI: 10.1016/j.psj.2023.102992
• Publication Date: 2023-08-05T01:49:54Z
• Authors: Wenjia Wang; Desheng Wu; Jinxue Ding; Jinli Wang; Jinwu Meng; Ke Ming; Siya Li; Tianxin Qiu; Jiaguo Liu; Danchen Aaron Yang
• Keywords: Lipopolysaccharides; SIRT1/PGC-1α; apoptosis; Apoptosis; SF1-1100; Animal culture; Mitochondria; Oxidative Stress; Sirtuin 1; chicken liver; inflammation; mitochondrial dysfunction; IMMUNOLOGY, HEALTH AND DISEASE; Hepatocytes; Animals; Chickens; Signal Transduction

The modified rougan decoction (MRGD) compound formula has been proven a certain ability to relieve lipopolysaccharide-enrofloxacin (LPS-ENR)-induced liver oxidant injury in chickens. Recent advances have shown that mitochondrial dysfunction affects the development of many diseases, leading increased interest exploring its effects. Using LPS-ENR-injured vivo and vitro further evaluate effects MRGD on structure function, emphasized investigation molecular mechanism. After LPS-ENR treatment, levels inflammation apoptosis markers were increased, along with higher injury. Results showed reduced inflammatory factors expression inhibited nuclear translocation NF-κB P65, reducing response vitro. Additionally, pretreatment dysfunction, oxidative stress, pathway by maintaining function. Moreover, treatment inhibitor EX527 promoted biogenesis through SIRT1/PGC-1α interfered dynamics, activate Nrf2. In summary, played key role promoting function thus alleviating hepatocyte at least part.