Molecular detection of multidrug-resistant Pseudomonas aeruginosa of different avian sources with pathogenicity testing and in vitro evaluation of antibacterial efficacy of silver nanoparticles against multidrug-resistant P. aeruginosa

• DOI: 10.1016/j.psj.2023.102995
• Publication Date: 2023-08-03T00:54:14Z
• Authors: Sherif Marouf; Xiting Li; Heba M. Salem; Zeinab S. Ahmed; Sara M. Nader; Mohamed Shaalan; Fatma H. Awad; Hongyan Zhou; Tuckyun Cheang
• Keywords: Neomycin

Pseudomonas aeruginosa (P. aeruginosa) is a serious zoonotic pathogen threaten the poultry industry causing severe economic losses therefor, this study aimed to isolation, phenotypic, molecular identification of P. from different avian sources (chickens, turkey, pigeons, table eggs and dead in shell chicken embryos), Egyptian governorates (Giza, Qalubia, Beheira, El- Minya Al-Sharqia) with applying antibiotic sensitivity test on all isolates. Highly resistant isolates (n=49) were subjected detection genes including carbapenemase-encoding blaKPC, blaOXA-48, blaNDM. On base results, highly strain was tested for its pathogenicity day old Specific Pathogen Free (SPF) chicks. Also, in-vitro experiment adopted evaluate efficacy silver nanoparticles (Ag-NPs) against strains. The overall isolation percentage examined samples 36.2 % (571/1576) representing 45.2% (532/1176) birds' tissues 39/400 (9.7%) total egg samples. Some isolated strains showed multidrug resistance (MDR) kanamycin, amoxicillin, amoxicillin-clavulanic acid, neomycin, chloramphenicol, vancomycin, cefotaxime clavulanic lincomycin-spectinomycin, co-trimoxazole, cefoxitin, gentamycin doxycycline. These MDR also molecularly positive ESBL carbapenemase encoding genes. high histopathological alterations organs challenged birds. Main lesions necrosis hepatocytes, renal tubular epithelium, heart muscle bundles. Ag-NPs. In conclusion, morbidity, mortality, pathological tissue alterations. Ag NPs revealed promising antimicrobial further vivo studies recommended.