Improving the quality of chilled semen from Thai native chicken using phosphorus and vitamin B12 supplementation in semen extender

• DOI: 10.1016/j.psj.2023.103262
• Publication Date: 2023-11-07T03:53:55Z
• Authors: Junpen Suwimontee...; Unchean Yamsrikaew; Khemiga Damthongsen; Thornjutha Suksir...; Paniga Leeniwa; Pawita Lawanyakul; Morakot Nuntapaitoon
• Keywords: Semen collection; Semen extender; Semen quality; Rooster; Membrane integrity

This study aimed to determine phosphorus and vitamin B12 supplementation effect in semen extender on the quality fertility ability of chilled Thai native rooster semen. Eighty-four ejaculates from 26 roosters (Burmese x Vietnam crossbreed) were included. Semen was collected by applying dorsal–abdominal massage once a week, pooled, diluted 500 million sperms per dose, divided into six groups. The samples used for control group with modified Beltsville poultry (BPSE). For treatment groups 2–6: BPSE enriched (Octafos® Octa Memorial Co., Ltd., Bangkok, Thailand) at concentrations 0.02%, 0.04%, 0.06%, 0.08%, 0.10%. tested replications inseminating layer hens. Thirty-six hens randomly assigned three (control, 0.08%) 12 inseminated dose 0.2 mL collecting day. Sperm motion characteristics (i.e., sperm motility, progressive kinetic parameters) measured using computer-assisted analysis system (SCA®, Proiser S.L., Valencia, Spain). viability, mitochondrial activity, acrosome integrity, plasma membrane malondialdehyde (MDA) concentration also evaluated. highest 0.04% all days collection, especially VCL VAP (P < 0.05). integrity spermatozoa greater than had lowest MDA collection. higher both (66.59% vs. 48.50%: P 0.05) hatching rates (58.80% 43.18%: group. In conclusion, improved