Molecular characterization of Campylobacter spp. isolates obtained from commercial broilers and native chickens in Southern Thailand using whole genome sequencing

• DOI: 10.1016/j.psj.2024.103485
• Publication Date: 2024-01-21T04:45:35Z
• Authors: Doan Hoang Phu; Tuempong Wongtawan; Phitchayapak Wint...; Nguyen Thi Nhung; Nguyen Thi Phuong...; Juan Carrique-Mas; Conny Turni; Lida Omaleki; Patrick J. Blackall; Thotsapol Thomron...
• Keywords: 0301 basic medicine; whole genome sequencing; Whole Genome Sequencing; MICROBIOLOGY AND FOOD SAFETY; Campylobacter; Campylobacter coli; Microbial Sensitivity Tests; bioinformatic tool; Thailand; SF1-1100; Animal culture; Anti-Bacterial Agents; Campylobacter jejuni; 03 medical and health sciences; Anti-Infective Agents; Campylobacter Infections; Drug Resistance, Bacterial; Animals; Humans; Tylosin; antimicrobial resistance; Chickens

Chickens are the primary reservoirs of Campylobacter spp., mainly C. jejuni and coli, that cause human bacterial gastrointestinal infections. However, genomic characteristics antimicrobial resistance spp. in low- to middle-income countries need more comprehensive exploration. This study aimed characterize 21 5 coli isolates from commercial broilers native chickens using whole genome sequencing compare them 28 reference sequences. Among 26 isolates, 13 sequence types (ST) were identified ST coli. The prominent was 2274 (5 19.2%), followed by 51, 460, 2409, 6455 (2 each ST, 7.7%), while all remaining (464, 536, 595, 2083, 6736, 6964, 8096, 10437, 828, 872, 900, 8237, 13540) had 1 isolate per (3.8%). Six genes (ant(6)-Ia, aph(3')-III, blaOXA, cat, erm(B), tet(O)) one point mutations gyrA gene (Threonine-86-Isoleucine) another rpsL (Lysine-43-Arginine) detected. blaOXA present 95.2% 80.0% tet(O) 76.2% Additionally, 203 virulence-associated linked 16 virulence factors identified. In terms phenotypic resistance, resistant ciprofloxacin, enrofloxacin, nalidixic acid, with lower levels tetracycline (76.2%), tylosin (52.3%), erythromycin (23.8%), azithromycin (22.2%), gentamicin (11.1%). Most tested antimicrobials, pan-susceptible except for tylosin. Single-nucleotide polymorphisms concordance varied widely, differences up 13,375 single-nucleotide compared highlighting genetic divergence among comparative genomes. contributes a deeper understanding molecular epidemiology Thai chicken production systems.