Integrating metabolomics and transcriptomics to analyze the differences of breast muscle quality and flavor formation between Daweishan mini chicken and broiler

• DOI: 10.1016/j.psj.2024.103920
• Publication Date: 2024-05-31T16:43:03Z
• Authors: Jingying Zhao; Xuehai Ge; Tao Li; Min Yang; Ruohan Zhao; Shixiong Yan; Hao Wu; Yong Liu; Kun Wang; Zhiqiang Xu; Junjing Jia; Lixian Liu; Tengfei Dou
• Keywords: Intramuscular fat; Anserine

The quality and flavor of chicken are affected by muscle metabolites related regulatory genes, the molecular regulation mechanism meat is different among breeds chicken. In this study, 40 one-day-old Daweishan mini (DM) Cobb broiler (CB) were selected from each group, with 4 replicates 10 chickens in replicate. reared until 90 d age under same management conditions. Then, metabolomics transcriptomics data 90-day-old DM (n = 4) CB integrated to analyze affecting breast flavor, explore important genes regulating metabolites. results showed that a total 38 significantly (SDMs) 420 differentially expressed (DEGs) detected 2 breeds. Amino acid lipid metabolism may be cause difference between chickens, involving such as L-methionine, betaine, N6, N6-Trimethyl-L-lysine, L-anserine, glutathione, glutathione disulfide, L-threonine, N-Acetyl-L-aspartic acid, succinate, choline, DOPC, SOPC, alpha-linolenic L-palmitoylcarnitine, etc. Important high correlation amino acids (GATM, GSTO1) lipids (PPARG, LPL, PLIN1, SCD, ANGPTL4, FABP7, GK, B4GALT6, UGT8, PLPP4) identified analysis, gene-metabolite interaction network mass formation was constructed. This study there significant differences mainly These kinds substances main reasons for general, could provide theoretical basis further research on reference development, utilization genetic breeding high-quality