Lactobacillus reuteri alleviates LPS-induced intestinal mucosal damage by stimulating the expansion of intestinal stem cells via activation of the Wnt/β-catenin signaling pathway in broilers

• DOI: 10.1016/j.psj.2024.104072
• Publication Date: 2024-07-09T00:19:03Z
• Authors: Xiaoqing Ding; Runzi Tang; Jiayue Zhao; Yibin Xu; Aikun Fu; Xiuan Zhan
• Keywords: Intestinal epithelium; Lactobacillus reuteri; Intestinal mucosa; Barrier function; LGR5; Intestinal Disorder; Ex vivo

The continuous expansion of intestinal stem cells (ISCs) is crucial for maintaining the renewal epithelium, particularly in inflammatory conditions. It remains largely unknown how internal microbiota repair damage to mucosal barrier. Hence, investigating potential anti-inflammatory probiotics from symbolic microbes broilers and analyzing their mechanism action support barrier function can offer novel regulatory tools alleviate broiler enteritis. In this research, we utilized vivo plus ex organoids model thoroughly examine effectiveness Lactobacillus reuteri (LR) protecting integrity mucosa during lipopolysaccharide-induced (LPS-induced) enteritis broilers. findings indicated that LR feeding maintained morphological structural integrity, enhanced proliferation epithelial cells, inhibited cell apoptosis response against deleterious effects triggered by LPS. Simultaneously, ISCs activity stimulated regeneration protect LPS-induced injury coculture system ileum revealed increased growth attenuated LPS-stimulated organoids. Furthermore, decrease ISC was rescued reactivation Wnt/β-catenin signaling vivo. This research promoted regulating pathway, thereby finding provided theoretical lactobacillus as a probiotic additive livestock feed improve inflammation treat diseases.