LeishGEM: genome-wide deletion mutant fitness and protein localisations in Leishmania

• DOI: 10.1016/j.pt.2024.06.003
• Publication Date: 2024-07-18T12:08:12Z
• Authors: Sidonie Aellig; Karen Billington; Jeziel D. Damasceno; Laura Davidson; Ulrich Dobramysl; Ruth Etzensperger; Eden Ramalho Ferr...; Eva Gluenz; Jeremy C. Mottram; Rachel Neish; Raquel Pereira; James Smith; Jack D. Sunter; Petr Volf; Richard J. Wheeler; Matthew Young
• Keywords: Leishmania; Proteomics; Leishmania mexicana; Protozoan Proteins; Genetic Fitness; Genome, Protozoan; Gene Deletion

LeishGEM is a genome-wide functional annotation community resource for Leishmania mexicana, where deletion mutant growth in vitro and in vivo is measured and protein localisation is determined by endogenous tagging and LOPIT-DC (localisation of organelle proteins by isotope tagging with differential centrifugation) spatial proteomics. Data are being made available pre-publication via http://leishgem.org which allows data-driven identification of the mechanisms for Leishmania parasitism.