Optimum gamma irradiation doses for mutagenesis in Bambara groundnut (Vigna subterranean L.) genotypes

• DOI: 10.1016/j.repbre.2024.01.002
• Publication Date: 2024-02-26T22:30:07Z
• Authors: Maliata Athon Wanga; Ruusa Napandulwe ...; Rose-mary Kavemui...; Annethe Kangumba; Magdalena Ndafapa...; Eddie B.S. Hasheela; Fatma Sarsu; Hussein Shimelis
• Keywords: Gamma Irradiation

Genetic diversity enhancement to select Bambara groundnut (Vigna subterranea [L.] Verdc. 2x = 2n 22) breeding lines with economic traits is imperative improve the crop's utilization in Sub-Saharan Africa. Gamma radiation a vital mutagenic agent create novel allelic combinations required for developing climate-smart varieties contribute food production and nutrition security drier regions, including Namibia. Therefore, objective of present study was identify optimal gamma doses enhance genetic genotypes. The conducted root boxes under custom-made shade-net house at Mannheim Crop Research Station, Tsumeb, Three genotypes (Uniswa Red, Dip C KFBN 9709) were irradiated six (0, 50, 100, 150, 200, 250 Gy) laid out randomized complete block design (RCBD) three replications. Data collected on days emergence (DTE), percentage (%EM), seedling survival (%SS), length (SLT), shoot weight (STW). A non-significant interaction genotype × dose detected, showing stable responses indicating that require varying mutagenesis. significant (p < 0.01) effect found emergence, percentage, weight, requirements individual varied. Significant 0.05) effects revealed could be used optimum upscale induced mutation programs groundnut. Seedling only trait higher growth reduction resulted negative trends. linear regression model predicted LD50s Uniswa KBFN 9709 428.1, 523.7 712.5 GY, respectively. These than test doses, need resulting LD50s. identified these studies are useful use tested