Fibronectin-coating enhances attachment and proliferation of mesenchymal stem cells on a polyurethane meniscal scaffold

• DOI: 10.1016/j.reth.2021.11.001
• Publication Date: 2021-11-26T22:58:54Z
• Authors: Raquel Arredondo; Francesco Poggioli; Santos Martínez-Díaz; María Piera-Trilla; Raúl Torres-Clara...; Laura Tío; Joan C. Monllau
• Keywords: CMI, collagen meniscal implant; Medicine (General); PCL-PU, polycaprolactone-polyurethane; MSCs, mesenchymal stem cells; ITS, Insulin Transferrin Selenium; 03 medical and health sciences; R5-920; 0302 clinical medicine; Tissue engineering; Rbm, rabbit bone marrow; rBM, rabbit bone marrow; rCHs, rabbit chondrocytes; Fibronectin; FN, fibronectin; Mesenchymal stem cell; Rchs, rabbit chondrocytes; Scaffolds; PSR, picrosirius red; QH573-671; Post-meniscectomy syndrome; AMT, allograft meniscus transplantation; ECM, extracellular matrix; MNCs, mononuclear cells; RT, room temperature; Original Article; Cytology; Meniscal injuries

Partial meniscectomy is one of the most common surgical strategy for a meniscal injury, but sometimes, patients complain knee pain due to an overload in ablated compartment. In these cases, implantation tissue engineering scaffold could be indicated. Currently, two commercial scaffolds, based on collagen or polycaprolactone-polyurethane (PCL-PU), are available meniscus scaffolding. short term follow-up assessments, both showed clinical improvement and formation. However, long-term studies carried out PCL-PU that new decreased volume assumed irregular shape. Moreover, some was totally reabsorbed, without formation.Mesenchymal stem cells (MSCs) combined with scaffolds represents promising approach treating defects because their multipotency self-renewal. this work, we aimed compare behaviour MSCs chondrocytes vitro. express integrins binds fibronectin (FN), so also investigate effect FN coating bioactivity scaffold. We isolated rabbit bone marrow (rBM-MSCs) from skeletally mature New Zealand white rabbits stablished optimum culture condition expand them. Then, they were seeded over non-coated FN-coated cultured chondrogenic conditions. To evaluate cell functionality, performed MTS assay proliferation between Finally, histologic study assess extracellular matrix (ECM) production samples, them ones obtained (rCHs) A protocol low FBS concentration set as best rBM-MSCs expansion. The revealed have more (145%; 95% CI: 107%-182%) compared scaffolds. demonstrated rCHs displayed highest ECM, followed by Furthermore, types produced comparable ECM pattern. These results suggest capacity attachment presence integrin alpha5beta1 (FN-receptor) allows interact applied design might important implications orthopaedic surgery injuries.