Development of triple-functionalized calcium phosphate nanoparticles as an advanced drug delivery system for bone tissue repair

• DOI: 10.1016/j.reth.2023.11.010
• Publication Date: 2023-11-28T06:01:50Z
• Authors: Taichi Tenkumo; Benedikt Kruse; Kathrin Kostka; Viktoriya Sokolova; Toru Ogawa; Nobuhiro Yoda; Oleg Prymak; Osamu Suzuki; Keiichi Sasaki; Matthias Epple
• Keywords: RUNX2; Bone tissue

During tissue repair or regeneration, several bioactive molecules are released and interact with each other act as complex additives inhibitors for reconstruction. In this study, the bone-healing effects of combination treatment tumor necrosis factor-α (TNF-α) inhibition, vascular endothelial growth factor A (VEGF-A) bone morphogenetic protein-7 (BMP-7) release by gene silencing, transfection calcium phosphate nanoparticles (CaP) in rat femoral head was histologically, morphologically, biochemically evaluated.A triple-functionalized paste CaP carrying plasmid DNA encoding BMP-7 VEGF), siRNA against TNF-α developed denoted CaP3mix. To compare 3mixCaP, BMP-7, VEGF, prepared CaP/PEI/pBMP-7/SiO2, CaP/PEI/pVEGF/SiO2, CaP/PEI/siRNA-TNF-α/SiO2, respectively. The healing defects investigated after 10 21 days implantation.The levels formation-related markers OCN, Runx2, SP7 increased at protein 3mixCaP days, significantly accelerated compared treatments loading VEGF is a promising material repair.