Evaluation of the effect of co-transplantation of collagen-hydroxyapatite bio-scaffold containing nanolycopene and human endometrial mesenchymal stem cell derived exosomes to regenerate bone in rat critical size calvarial defect

• DOI: 10.1016/j.reth.2024.02.006
• Publication Date: 2024-07-02T07:38:17Z
• Authors: Masoumeh Einabadi; Azadeh Izadyari A...; Laleh Foroutani; Arman Ai; Mojdeh Salehi Namini; Ali Farzin; Amir Nahanmoghadam; Sadegh Shirian; Hossein Kargar Ja...; Jafar Ai
• Keywords: Exosome

This study aimed to evaluate the effect of nanoparticles based on PLGA and biomolecule lycopene (i.e. NLcp) exosomes loaded hydroxyapatite/collagen-based scaffolds (HA/Coll), human endometrial MSCs (hEnMSCs) differentiation into osteoblast cells. To this end, after synthesizing NLcp isolating hEnMSC-derived exosomes, studying their characterizations, HA/Coll scaffold with/without exosome was fabricated. In following, rat skull-defect model created 54 male Sprague–Dawley rats (12 weeks old) which were classified 6 groups [control group (4 healthy rats), negative control group: bone defect without grafting (10 experimental including grafted with HA/Coll/NLcp + HA/Coll-NLcp rats)]. Finally, membrane along its surrounding tissues removed at 90 days surgery, assess amount repair by Hematoxylin eosin staining. Moreover, immunohistochemical X-ray Micro-Computed Tomography (Micro-CT) analyses performed osteocalcin mean volume fraction (BVF). Based results, although, existence in network can significantly increase BVF compared (2.25-fold 1.5-fold, respectively). However, combination indicated more BVF; so that led a 15.95 % than exosome. Hence, synthesized act as suitable bioactive stimulate osteogenic, promotion cell proliferation when used polymer structure or polymeric carriers containing