Enrichment of low-abundance proteins from bovine and porcine serum samples for proteomic studies

• DOI: 10.1016/j.rvsc.2010.03.019
• Publication Date: 2010-04-05T08:20:03Z
• Authors: Anna Marco-Ramell; Anna Bassols
• Keywords: Proteomics; 0301 basic medicine; 570; 0303 health sciences; Swine; Reproducibility of Results; Blood Proteins; name=SDG 3 - Good Health and Well-being; 3. Good health; 03 medical and health sciences; Immunoglobulin G; /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being; Animals; Cattle; Electrophoresis, Gel, Two-Dimensional; Serum Albumin

One of the main applications of serum proteomics is the identification of new biomarkers for animal disease or animal production. However, potential obstacles to these studies are the poor performance of affinity serum depletion methods based on human antigens when using animal samples, and loss of minor serum components bound to albumin and other proteins. In the present study, we have analyzed the efficiency and reproducibility of the ProteoMiner® beads with bovine and porcine serum samples, and compared to a traditional immunoaffinity-based albumin and IgG depletion system specific for human samples. The ProteoMiner kit is based on the use of a combinatorial peptide binding library and intends to enrich low-abundance proteins.