Transforming growth factor-β transiently induces vimentin expression and invasive capacity in a canine mammary gland tumor cell line

0301 basic medicine Time Factors Mammary Neoplasms, Animal 03 medical and health sciences Dogs Mammary Glands, Animal Transforming Growth Factor beta Cell Line, Tumor Animals Vimentin Female Neoplasm Invasiveness Dog Diseases
DOI: 10.1016/j.rvsc.2012.10.016 Publication Date: 2012-11-23T11:31:21Z
ABSTRACT
The epithelial-mesenchymal transition (EMT) is a crucial event that occurs during cancer metastasis and can be induced by transforming growth factor-β (TGF-β) in various tumor cells in vitro. However, little is known about the effects of TGF-β in canine mammary gland tumors (CMGTs). Here, we investigated the role of TGF-β in CMGT. We observed that treatment of the CMGT cell line CHMp13a with TGF-β1 leads to transient induction of the mesenchymal marker vimentin. Real-time measurements of cellular electrical impedance also showed that CMGT invasiveness is transiently increased by TGF-β1 treatment, but is reversed after prolonged stimulation. This phenomenon is similar to the mesenchymal-epithelial transition (MET, the reverse phenomenon of EMT), and a process that is implicated in the establishment of secondary metastatic lesions.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (23)
CITATIONS (13)