Transforming growth factor-β transiently induces vimentin expression and invasive capacity in a canine mammary gland tumor cell line
0301 basic medicine
Time Factors
Mammary Neoplasms, Animal
03 medical and health sciences
Dogs
Mammary Glands, Animal
Transforming Growth Factor beta
Cell Line, Tumor
Animals
Vimentin
Female
Neoplasm Invasiveness
Dog Diseases
DOI:
10.1016/j.rvsc.2012.10.016
Publication Date:
2012-11-23T11:31:21Z
AUTHORS (8)
ABSTRACT
The epithelial-mesenchymal transition (EMT) is a crucial event that occurs during cancer metastasis and can be induced by transforming growth factor-β (TGF-β) in various tumor cells in vitro. However, little is known about the effects of TGF-β in canine mammary gland tumors (CMGTs). Here, we investigated the role of TGF-β in CMGT. We observed that treatment of the CMGT cell line CHMp13a with TGF-β1 leads to transient induction of the mesenchymal marker vimentin. Real-time measurements of cellular electrical impedance also showed that CMGT invasiveness is transiently increased by TGF-β1 treatment, but is reversed after prolonged stimulation. This phenomenon is similar to the mesenchymal-epithelial transition (MET, the reverse phenomenon of EMT), and a process that is implicated in the establishment of secondary metastatic lesions.
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CITATIONS (13)
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