Effect of brassinosteroids on drought resistance and abscisic acid concentration in tomato under water stress

• DOI: 10.1016/j.scienta.2010.06.014
• Publication Date: 2010-07-22T08:37:49Z
• Authors: Gao-Feng Yuan; Cheng-Guo Jia; Zhen Li; Bo Sun; Li-Ping Zhang; Na Liu; Qiao-Mei Wang
• Keywords: APX; Brassinolide; Malondialdehyde; Brassinosteroid; Stomatal Conductance; Lycopersicon; Drought Tolerance

Abstract The effect of brassinosteroid (BR) on relative water content (RWC), stomatal conductance (g s ), net photosynthetic rate ( P N), intercellular CO 2 concentration (Ci), lipid peroxidation level, activities of antioxidant enzymes and abscisic acid concentration (ABA) in tomato ( Lycopersicon esculentum ) seedlings under water stress was investigated. Two tomato genotypes, Mill. cv. Ailsa Craig (AC) and its ABA-deficient mutant notabilis ( not ), were used. Water stress was achieved by withholding water and both the AC and not plants were treated with 1 μM 24-epibrassinolide (EBR) or distilled water as a control. The RWC, g s , Ci and P N were significantly decreased under water stress. However, EBR treatment significantly alleviated water stress and increased the RWC and P N. EBR application also markedly increased the activities of antioxidant enzymes (catalase, ascorbate peroxidase and superoxide dismutase) while it decreased g s , Ci and the contents of H 2 O 2 and malondialdehyde (MDA). Interestingly, ABA concentration in AC and not plants was markedly elevated after EBR treatment although the increasing rate and amplitude of ABA in not plants treated by EBR was significantly lower than those in AC plants. Our study suggested that amelioration of the drought stress of tomato seedlings may be caused by EBR-induced elevation of endogenous ABA concentration and/or the activities of antioxidant enzymes.