Arabinogalactan protein is a molecular and cytological marker of particular stages of the tomato fruit ripening process

• DOI: 10.1016/j.scienta.2022.111718
• Publication Date: 2022-11-26T04:40:00Z
• Authors: Nataliia Kutyriev...; Agata Leszczuk; Adrian Zając; Panagiotis Kalaitzis; Artur Zdunek
• Keywords: Arabinogalactan; Molecular mass; Molecular marker

Arabinogalactan proteins (AGPs) are constituents of the plant cell walls, which increasingly being considered as essential molecules in formation extracellular matrix. Given their molecular structure and advantage carbohydrate moiety, glycosylation level its modification is assumed a cause functional diversity AGPs. The aim current work was to evaluate features AGP potential molecule involved ripening process. For this purpose, tomato fruits were analyzed at specific five stages using microscopic tools. experiment showed that high content occurrence with weight around 120 kDa related wall conditions intensity ongoing glycosylation. At breaker and/or turning stage, processes sugar moiety depolymerization begin prevail. red ripe/pink synthesis process replaced by degradation process, associated disappearance AGPs weights appearance only single residues very low weights. (∼30 kDa) may be used marker finalization fruits. All anatomical morphological alterations confirm presence dependencies connections between components network. We presume selected antibodies (JIM13, LM2, LM14) indicate fruit tissue different ripening; thus, cytological particular