Genomic characterization of BKC-1–producing Klebsiella pneumoniae strain belonging to high-risk clone sequence type 11 isolated from a river in Brazil

• DOI: 10.1016/j.scitotenv.2022.157917
• Publication Date: 2022-08-08T21:07:33Z
• Authors: Amalia Lobato; Cintya O. Souza; Willames M.B.S. M...; Rafael R. Barata; Daniel S. Camargo; Lívia M.G. Dutra; Irna C.R.S. Carneiro; Clevia J.S. Costa; Danielle M. Brasi...
• Keywords: Siderophores; Genomics; Microbial Sensitivity Tests; Type VI Secretion Systems; beta-Lactamases; Anti-Bacterial Agents; Clone Cells; Klebsiella Infections; 3. Good health; Klebsiella pneumoniae; Aminoglycosides; Bacterial Proteins; Carbapenems; Rivers; Drug Resistance, Multiple, Bacterial; Klebsiella; Humans; Polymyxins; Brazil; Fluoroquinolones; Plasmids

Carbapenem-resistant Klebsiella pneumoniae is a common cause of healthcare-related infections, and it is widespread in hospitals and diverse environments with potentially serious public health implications. Herein, we have reported the isolation and characterization of an environmental Brazilian Klebsiella carbapenemase (BKC-1)-producing K. pneumoniae strain (IEC1205) isolated in 2018 from a river in the Amazon region, Brazil. Antimicrobial susceptibility of this strain was evaluated by broth microdilution and demonstrated resistance to several antibiotics including β-lactams, aminoglycosides, fluoroquinolones, and polymyxins. It has an extensively drug-resistant phenotype. Genomic analysis revealed that IEC1205 belonged to sequence type 11, clonal complex 258 and the presence of blaBKC-1 and two other β-lactamase-encoding genes (blaCTX-M-15 and blaSHV-11). The predicted virulence was associated with biofilm formation-related genes, a type VI secretion system, siderophore production, and type I and II fimbriae formation. We have identified an IncQ1 plasmid, named pIEC1205, harboring blaBKC-1 with high similarity to previously described plasmids carrying blaBKC-1 and blaBKC-2 genes. To our knowledge, this is the first report of an environmental BKC-1-producing K. pneumoniae strain.