Cells with surface expression of CD133highCD71low are enriched for tripotent colony-forming progenitor cells in the adult murine pancreas

• DOI: 10.1016/j.scr.2015.11.015
• Publication Date: 2015-12-01T03:24:06Z
• Authors: Liang Jin; Dan Gao; Tao Feng; Jacob R. Tremblay; Nadiah Ghazalli; Angela Luo; Jeffrey Rawson; Janine C. Quijano; Jing Chai; Lena Wedeken; Jasper Hsu; Jeanne LeBon; Stephanie Walker; Hung-Ping Shih; Alborz Mahdavi; David A. Tirrell; Arthur D. Riggs; H. Teresa Ku
• Keywords: Progenitor

Progenitor cells in the adult pancreas are potential sources of endocrine beta for treating type 1 diabetes. Previously, we identified tri-potent progenitor (2-4month-old) murine that were capable self-renewal and differentiation into duct, acinar, vitro. These named pancreatic colony-forming units (PCFUs). However, because PCFUs a minor population (~1%) they difficult to study. To enrich PCFUs, strategies using cell-surface marker analyses fluorescence-activated cell sorting developed. We found CD133(high)CD71(low) cells, but not other populations, enriched by up 30 fold compared unsorted cells. generated primary, secondary, subsequent colonies when serially re-plated Matrigel-containing cultures, suggesting abilities. In presence laminin hydrogel, gave rise contained Insulin(+)Glucagon(+) double-hormonal Colonies from hydrogel culture implanted diabetic mice, five weeks later Insulin(+)Glucagon(-) detected grafts, demonstrating tri-lineage will enable future studies putative stem vivo.