Derivation of induced pluripotent stem cells line (RCPCMi007-A-1) with inactivation of the beta-2-microglobulin gene by CRISPR/Cas9 genome editing

• DOI: 10.1016/j.scr.2021.102451
• Publication Date: 2021-06-30T16:09:33Z
• Authors: M.E. Bogomiakova; E.K. Sekretova; A.V. Eremeev; L.D. Shuvalova; P.A. Bobrovsky; E.A. Zerkalenkova; O.S. Lebedeva; M.A. Lagarkova
• Keywords: Germ layer

The mismatch of HLA haplotypes between donor and recipient adversely affects the outcome tissue transplantation. TheB2Mgene knockout (B2M-KO) disrupts I heterodimer formation; therefore,B2M-KO cells have reduced immunogenicity to allogeneic CD8+ T cells. Thus, theB2M-KO IPSCs their derivatives can potentially solve a problem immunological compatibility in transplantations. Using CRISPR/Cas9-mediated genome editing, we generated human B2M-KO iPSC line (RCPCMi007-A-1). RCPCMi007-A-1 iPSCs express pluripotency markers, typical stem cell morphology, maintain normal karyotype, ability differentiate into three germ layers.