Development of efficient strain of Ganoderma lucidum for biological stripping of cotton fabric dyed Reactive Blue 21

• DOI: 10.1016/j.sjbs.2021.09.038
• Publication Date: 2021-09-17T15:15:38Z
• Authors: Zainab Latif; Javeria Shafique; Baby Summuna; Bashir Lone; Munib ur Rehman; Mohamed A. El-Sheikh; Maha J. Hashim; Carmen Vladulescu; Tahira Shafique
• Keywords: Textile; Central composite design; Stripping (fiber); Woven fabric

One of the most common dyeing problems textile industries is uneven and faulty over finished quality fabrics due to different reasons. These are usually tackled through chemical degradation in which dye removed from surface fiber but fabric compromised. Chemical process also reduces strength durability material by reduction reactive ability. The cannot be reused reduced strength. To overcome above mentioned problem, biological method stripping enzymes produced micro-organisms used. This has no harmful effect on safe for environment. In this research work blue 21 with 0.5, 2 4% shade strengths was used cotton fabric. Ganoderma lucidum fungal strains were mutated UV mutagen, five selected further processing. mutant grown at temperature ranges (20 °C 40 °C); pH(3-5); inoculum size(1-5 mL) fermentation time (3-15 days) . required nutrients media produce ligninolytic added flask. strain gave fast decolourization results optimization. Optimization done observing variables: incubation 12 days, pH 4, 30 °C, size 3 mL applying Response Surface Methodology (RSM) Central Composite Design (CCD). During color stripping, enzyme assay revealed that respective UV-60 active their Vmax, Mnp (427U/mL), LiP (785U/mL), Lac (75 U/mL) decolorized 89% 25% more than parent production (344U/mL), (693U/mL), (59 lower strain.