One-step synthesis of uniform nanoparticles of porphyrin functionalized ceria with promising peroxidase mimetics for H2O2 and glucose colorimetric detection

• DOI: 10.1016/j.snb.2016.09.049
• Publication Date: 2016-09-12T19:37:57Z
• Authors: Qingyun Liu; Yanting Yang; Xintian Lv; Yanan Ding; Yaozu Zhang; Jingjing Jing; Chengxin Xu
• Keywords: Glucose oxidase; Linear range

Abstract Hydrogen peroxide (H 2 O 2 ) is a key molecule in biology. As a byproduct of many enzymatic reactions, H 2 O 2 is also a popular biosensor target. We report the first use of uniform nanoparticles of porphyrin functionalized ceria (Por-Ceria) prepared by a one-step method, as a colorimetric probe in detection for H 2 O 2 . Por-ceria nanoparticles exhibited strong intrinsic peroxidase activity toward a classical peroxidase substrate, 3,3′,5,5′-tetramethylbenzidine (TMB), in the presence of H 2 O 2 . Enhanced peroxidase-like activity of Por-ceria originated from synergistic effect of porphyrin and ceria, thereby explaining the high performance of Por-Ceria as an artificial enzyme mimicking peroxidase. When coupled with glucose oxidase, glucose is detected. A detection limit of 1.9 × 10 −2 mM glucose with a linear range up to 0.15 mM.