Self-assembled small molecule based fluorescent detection of serum albumin proteins: Clinical detection and cell imaging

• DOI: 10.1016/j.snb.2017.08.072
• Publication Date: 2017-08-10T16:50:05Z
• Authors: Prabhpreet Singh; Lalit Singh Mittal; Sandeep Kaur; Satwinderjit Kaur; Gaurav Bhargava; Subodh Kumar
• Keywords: Serum Albumin

Abstract We report perylenediimide-benzimidazolium based fluorescent ‘turn-on’ probe BIM-PDI for selective detection of human serum albumin (HSA) and bovine serum albumin (BSA) proteins. In HEPES buffer (0.1% DMSO), BIM-PDI self-assembles into aggregates and shows absorption maxima at 500 nm and weak fluorescence centered at 577 nm. The addition of HSA or BSA (1 × 10−9–5 × 10−8 M) to the solution of BIM-PDI results in decrease in the emission intensity at 577 nm. However, further increase in concentration of HSA/BSA results in appearance of new blue shifted emission band at 540 nm. The minimum detection limit for HSA/BSA is 3.01 × 10−10 M at 577 nm and 4.2 × 10−8 M at 540 nm. On addition of BSA to the solution of BIM-PDI, the size of the aggregates decreased from 100 to 250 nm to