Biotin-Yellow a biotin guided NIR turn-on fluorescent probe for cancer targeted diagnosis

• DOI: 10.1016/j.snb.2021.129807
• Publication Date: 2021-03-18T13:49:17Z
• Authors: Dimitrios A. Diam...; Adamantia Agalou; Maria V. Chatziat...; Georgios S. Marko...; Sofia Bellou; Zoi Kanaki; Timothy Crook; Nelofer Syed; Theodoros Rampias; Apostolos Klinakis; Evangelos Kolettas; Dimitris Beis; Andreas G. Tzakos
• Keywords: 01 natural sciences; 3. Good health; 0104 chemical sciences

Abstract A dicyanomethylene-4H-pyran based tumor-targeting and biothiol activatable fluorescent and colorimetric probe, named Biotin-Yellow, has been developed with unprecedented turn-on near-infrared fluorescence properties. Biotin-Yellow is non-toxic and can effectively recognize and discriminate human lung adenocarcinoma cells and urothelial (bladder) carcinoma cells from embryonic kidney cells and embryonic fibroblasts. Biotin-Yellow can also be employed for real-time bioimaging tracking of upregulated biothiol activity in cancer cells. The efficacy and biothiol responsiveness of Biotin-Yellow was further validated in vivo in zebrafish and mice xenografts.