Recovery and isolation of individual microfluidic picoliter droplets by triggered deposition

• DOI: 10.1016/j.snb.2022.132289
• Publication Date: 2022-06-28T19:25:53Z
• Authors: Thomas Weber; Sundar Hengoju; Ashkan Samimi; Martin Roth; Miguel Tovar; Miriam A. Rosenbaum
• Keywords: Microtiter plate; Interfacing; Petri dish; Isolation; Digital Microfluidics

Microfluidic emulsion-based droplet systems have a great potential for inexpensive ultrahigh-throughput experimentation. Yet, picking and upscaling single unique picoliter-sized droplets of interest out million others deeper analysis is still fundamental limitation. In order to overcome this missing gap, we present system in which are collected into collection chamber (DropLot) high throughput then slowly redirected an agar surface or microtiter plate via capillary tube passing through optical sensor before exiting. The signal each triggers positioning algorithm that ultimately places the flowing at defined position on Petri dish plate. Results indicate effective isolation giving rise colonies with recovery rate over 93%. possibility isolate individual provides critical feature interfacing microfluidics standard laboratory processing.