Phosphorescence lifetime measurements with sensor materials: Comparison of the four different detection platforms

• DOI: 10.1016/j.snb.2022.132496
• Publication Date: 2022-08-10T16:34:16Z
• Authors: Rajannya Sen; Liang Li; Alexander V. Zhdanov; Rafael D.L. Gaspar; Dmitri B. Papkovsky
• Keywords: Microsecond; Flash-lamp

Using several types of Pt-porphyrin based oxygen sensing materials and conditions, we evaluated comparatively four different detection platforms that perform phosphorescence lifetime (PLT) measurements in the microsecond time domain. The time-resolved fluorescence reader Victor 2, which utilises Rapid Lifetime Determination method Xe-flash lamp excitation, produced stable but inaccurate readings at PLT values < 15 µs, affected by flash-lamp afterglow sensor heterogeneity oxygenated conditions. luminescence spectrometer Cary Eclipse, multi-point decay method, was able to partly correct these interfering factors produce more accurate from mono- bi-exponential fits, however short were still afterglow. wide-field Tpx3Cam cam-era-based macroscope confocal laser-scanning FLIM/PLIM microscope, use short-pulse excitation (ns LED or ps diode laser pulses, respectively) TCSPC revealed presence short-lived components (1.5–6.0 µs) sample decays, have be considered calculations. imaging in-formative data, they operate with large volumes raw processed data. Overall, for each platform a number potential sources error measurement artefacts been revealed, can minimised careful optimisation settings validation alternative materials.