FRET with MoS2 nanosheets integrated CRISPR/Cas12a sensors for robust and visual food-borne parasites detection

• DOI: 10.1016/j.snb.2023.134493
• Publication Date: 2023-08-22T01:19:46Z
• Authors: Xiuqin Chen; Xiaolei Liu; Yao Yu; Haolu Wang; Chengyao Li; Isabelle Vallée; Mingyuan Liu; Lianjing Zhao; Xue Bai
• Keywords: Trans-activating crRNA

Recently, CRISPR/Cas associated biosensors have been shown to great potential in sensing applications due their high sensitivity and base resolution. However, the signal reporter system containing two organic fluorescent dye pair is limited by cost less stability. In contrast, functional nanomaterials exhibit robust stability, excellent optical properties low preparation cost, making them suitable reporters. this study, a MoS2 nanosheets (NSs) improved CRISPR/Cas12a-based biosensing platform was constructed for first time detect food-borne parasites. NSs were used as fluorescence quenchers single-stranded DNA (ssDNA) discriminated carrier construct system. The combination of recombinase polymerase amplification with modulated CRISPR/Cas12a helped achieve attomolar nucleic acid detection within 35 min. Moreover, results obtained using portable apparatus, enabling visual at point care. practical applicability successfully achieved through anisakis real samples. This study provides novel insights into exploring feasibility two-dimensional based system, well offers reliable tool on-site monitoring